SLX4
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [ISO]
- DNA double-strand break processing involved in repair via single-strand annealing [ISO]
- DNA repair [ISO]
- double-strand break repair via homologous recombination [ISO]
- nucleotide-excision repair [ISO]
- positive regulation of catalytic activity [ISO]
- response to intra-S DNA damage checkpoint signaling [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ERCC1
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [ISO]
- DNA recombination [IMP, ISO]
- DNA repair [IMP]
- UV protection [IMP]
- cell development [IMP]
- cell proliferation [IMP]
- cellular response to DNA damage stimulus [IMP]
- chromosome organization [IMP]
- double-strand break repair [IMP]
- germ cell development [IMP]
- interstrand cross-link repair [IMP]
- isotype switching [IMP]
- male gonad development [IMP]
- mitotic recombination [ISO]
- multicellular organism growth [IGI, IMP]
- multicellular organismal aging [IMP]
- negative regulation of telomere maintenance [ISO]
- nucleotide-excision repair [IMP, ISO]
- nucleotide-excision repair, DNA incision, 3'-to lesion [ISO]
- nucleotide-excision repair, DNA incision, 5'-to lesion [ISO]
- oogenesis [IMP]
- post-embryonic hemopoiesis [IMP]
- pyrimidine dimer repair by nucleotide-excision repair [IMP]
- replicative cell aging [IMP]
- response to X-ray [IMP]
- response to oxidative stress [ISO]
- spermatogenesis [IMP]
- syncytium formation [IMP]
Gene Ontology Molecular Function- TBP-class protein binding [IPI]
- TFIID-class transcription factor binding [IDA]
- damaged DNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- single-stranded DNA binding [ISO]
- single-stranded DNA endodeoxyribonuclease activity [ISO]
- structure-specific DNA binding [ISO]
- TBP-class protein binding [IPI]
- TFIID-class transcription factor binding [IDA]
- damaged DNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- single-stranded DNA binding [ISO]
- single-stranded DNA endodeoxyribonuclease activity [ISO]
- structure-specific DNA binding [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mouse SLX4 is a tumor suppressor that stimulates the activity of the nuclease XPF-ERCC1 in DNA crosslink repair.
SLX4 binds to three nucleases (XPF-ERCC1, MUS81-EME1, and SLX1), and its deficiency leads to genomic instability, sensitivity to DNA crosslinking agents, and Fanconi anemia. However, it is not understood how SLX4 and its associated nucleases act in DNA crosslink repair. Here, we uncover consequences of mouse Slx4 deficiency and reveal its function in DNA crosslink repair. Slx4-deficient mice develop epithelial ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLX4 ERCC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SLX4 ERCC1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1515240 | |
| ERCC1 SLX4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1515242 | |
| SLX4 ERCC1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID