BAIT

CDS1

SPCC18B5.11c
replication checkpoint kinase cds1
GO Process (3)
GO Function (3)
GO Component (3)
Schizosaccharomyces pombe (972h)
PREY

HSK1

cdc7, SPBC776.12c
Dbf4(Dfp1)-dependent protein kinase Hsk1
Schizosaccharomyces pombe (972h)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

Sabatinos SA, Green MD, Forsburg SL

Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse. We found that prolonged DNA synthesis occurs in cds1Δ and mrc1Δ checkpoint mutants in the presence of HU and continues after release. This is coincident with increased DNA damage measured by phosphorylated histone H2A in whole cells during release. High-resolution live-cell imaging shows ... [more]

Mol. Cell. Biol. Dec. 01, 2012; 32(24);4986-97 [Pubmed: 23045396]

Throughput

  • Low Throughput

Ontology Terms

  • resistance to chemicals (APO:0000087)
  • vegetative growth (APO:0000106)

Additional Notes

  • hsk1ts partly rescued the viability of cds1 mutant cells in HU

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HSK1 CDS1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
247601
HSK1 CDS1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
442624
HSK1 CDS1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
570126
HSK1 CDS1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
246653
HSK1 CDS1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
246652

Curated By

  • BioGRID