A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes.

Ward TA, McHugh PJ, Durant ST

Flap endonuclease 1 (FEN1) is a structure selective endonuclease required for proficient DNA replication and the repair of DNA damage. Cellularly active inhibitors of this enzyme have previously been shown to induce a DNA damage response and, ultimately, cell death. High-throughput screens of human cancer cell-lines identify colorectal and gastric cell-lines with microsatellite instability (MSI) as enriched for cellular sensitivity ... [more]

PLoS ONE Jun. 20, 2017; 12(6);e0179278 [Pubmed: 28628639]

Throughput

Low Throughput

Additional Notes

Cells with bait knockdown using shRNA, and inhibition of hit with olaparib had lower survival than control

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRCA2 PARP2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Mengwasser KE (2019)	Low	-	BioGRID	3656460

Curated By

BioGRID

Interaction Summary

BRCA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

H3 histone acetyltransferase activity [IDA]H4 histone acetyltransferase activity [IDA]gamma-tubulin binding [IPI]histone acetyltransferase activity [IDA]protease binding [IPI]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

PARP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IBA]protein binding [IPI]