BAIT
BDF1
chromatin-binding protein BDF1, L000000165, YLR399C
Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication
GO Process (8)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- DNA repair [IMP]
- chromatin organization involved in regulation of transcription [IMP]
- chromatin remodeling [IPI]
- negative regulation of heterochromatin assembly [IGI, IMP]
- positive regulation of histone exchange [IMP]
- regulation of chromatin silencing at silent mating-type cassette [IMP]
- regulation of chromatin silencing at telomere [IMP]
- snRNA transcription [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CDC55
TMR4, protein phosphatase 2A regulatory subunit CDC55, L000000282, S000029602, L000003191, YGL190C
Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; homolog of mammalian B55
GO Process (7)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- cytokinesis after mitosis checkpoint [IGI]
- negative regulation of exit from mitosis [IMP]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of protein localization to nucleus [IMP]
- positive regulation of transcription by transcription factor localization [IMP]
- protein dephosphorylation [IDA, IMP]
- regulation of mitotic cell cycle spindle assembly checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -2.51 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID