BAIT
SWI3
HAF2, TYE2, L000002251, YJL176C
Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
GO Process (6)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [IDA]
- carbon catabolite activation of transcription from RNA polymerase II promoter [IMP]
- cellular alcohol catabolic process [IMP]
- positive regulation of mating type switching [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- sucrose catabolic process [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
YPK1
SLI2, serine/threonine protein kinase YPK1, L000002541, YKL126W
Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
GO Process (7)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- eisosome assembly [IGI]
- glycerophospholipid metabolic process [IGI, IMP]
- negative regulation of phospholipid translocation [IMP]
- negative regulation of sphingolipid biosynthesis involved in cellular sphingolipid homeostasis [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, IMP]
- response to pheromone involved in conjugation with cellular fusion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -5.56 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID