BAIT
MEC1
ESR1, RAD31, SAD3, protein kinase MEC1, L000000586, S000029404, S000007656, YBR136W
Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres
GO Process (9)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [IMP]
- DNA recombination [IMP]
- DNA replication [IMP]
- histone phosphorylation [IGI, IMP]
- nucleobase-containing compound metabolic process [IGI]
- positive regulation of DNA-dependent DNA replication [IMP]
- reciprocal meiotic recombination [IMP]
- telomere maintenance [IDA]
- telomere maintenance via recombination [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
TPM1
tropomyosin TPM1, L000002328, YNL079C
Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
GO Process (14)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- actin filament bundle assembly [IGI]
- actin filament organization [TAS]
- actin filament reorganization [IMP]
- actin polymerization or depolymerization [TAS]
- budding cell apical bud growth [TAS]
- budding cell isotropic bud growth [TAS]
- establishment of cell polarity [TAS]
- exocytosis [TAS]
- filamentous growth [IMP]
- intracellular mRNA localization [TAS]
- mitochondrion inheritance [TAS]
- pseudohyphal growth [IMP]
- vacuole inheritance [TAS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -2.79 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID