DLD
Gene Ontology Biological Process
- branched-chain amino acid catabolic process [TAS]
- cellular metabolic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- lysine catabolic process [TAS]
- pyruvate metabolic process [TAS]
- regulation of acetyl-CoA biosynthetic process from pyruvate [TAS]
- small molecule metabolic process [TAS]
- tricarboxylic acid cycle [TAS]
Gene Ontology Cellular Component
RPA3
Gene Ontology Biological Process
- DNA recombinase assembly [TAS]
- DNA repair [TAS]
- DNA replication [IMP, TAS]
- DNA strand elongation involved in DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- base-excision repair [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IMP, TAS]
- mismatch repair [IMP]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IMP, TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.
Mitochondrial protein (MP) dysfunction has been linked to neurodegenerative disorders (NDs); however, the discovery of the molecular mechanisms underlying NDs has been impeded by the limited characterization of interactions governing MP function. Here, using mass spectrometry (MS)-based analysis of 210 affinity-purified mitochondrial (mt) fractions isolated from 27 epitope-tagged human ND-linked MPs in HEK293 cells, we report a high-confidence MP network ... [more]
Quantitative Score
- 0.00574191 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- HEK293 cell line (FLAG TAGGED; AP/MS)
- Normalized Spectral Abundance Factor (NSAF) scores were associated with each mitochondrial protein (MP) interaction. High confidence MP interactions were filtered at a confidence threshold of 90%.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RPA3 DLD | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3637834 |
Curated By
- BioGRID