DLD
Gene Ontology Biological Process
- branched-chain amino acid catabolic process [TAS]
- cellular metabolic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- lysine catabolic process [TAS]
- pyruvate metabolic process [TAS]
- regulation of acetyl-CoA biosynthetic process from pyruvate [TAS]
- small molecule metabolic process [TAS]
- tricarboxylic acid cycle [TAS]
Gene Ontology Cellular Component
SMC3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.
Mitochondrial protein (MP) dysfunction has been linked to neurodegenerative disorders (NDs); however, the discovery of the molecular mechanisms underlying NDs has been impeded by the limited characterization of interactions governing MP function. Here, using mass spectrometry (MS)-based analysis of 210 affinity-purified mitochondrial (mt) fractions isolated from 27 epitope-tagged human ND-linked MPs in HEK293 cells, we report a high-confidence MP network ... [more]
Quantitative Score
- 8.15555e-05 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- HEK293 cell line (Antibody; IP/MS)
- HEK293 cell line (FLAG TAGGED; AP/MS)
- Normalized Spectral Abundance Factor (NSAF) scores were associated with each mitochondrial protein (MP) interaction. High confidence MP interactions were filtered at a confidence threshold of 90%.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DLD SMC3 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3680501 |
Curated By
- BioGRID