BAIT

BZZ1

LSB7, YHR114W
SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
GO Process (4)
GO Function (1)
GO Component (4)
Saccharomyces cerevisiae (S288c)
PREY

SIP1

L000001890, YDR422C
Alternate beta-subunit of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions
GO Process (3)
GO Function (1)
GO Component (2)
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A comprehensive two-hybrid analysis to explore the yeast protein interactome.

Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y

Protein-protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional interpretation of fully sequenced genomes, which are flooded with novel genes of unpredictable functions. We previously developed a system to examine two-hybrid interactions in all possible combinations between the approximately 6,000 proteins of the budding yeast Saccharomyces cerevisiae. ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 10, 2001; 98(8);4569-74 [Pubmed: 11283351]

Throughput

  • High Throughput

Curated By

  • BioGRID