BAIT
AKT1
Akt, PKB, PKB/Akt, PKBalpha, Rac
thymoma viral proto-oncogene 1
GO Process (82)
GO Function (15)
GO Component (12)
Gene Ontology Biological Process
- activation-induced cell death of T cells [ISO]
- aging [ISO]
- anagen [IMP]
- apoptotic mitochondrial changes [IDA]
- cell projection organization [ISO]
- cellular response to epidermal growth factor stimulus [IDA]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [IDA]
- cellular response to growth factor stimulus [IDA]
- cellular response to hypoxia [ISO]
- cellular response to insulin stimulus [IDA, IMP, ISO]
- cellular response to mechanical stimulus [ISO]
- cellular response to organic cyclic compound [ISO]
- cellular response to peptide [IDA]
- cellular response to prostaglandin E stimulus [IDA]
- cytoskeleton organization [TAS]
- execution phase of apoptosis [IDA]
- germ cell development [IDA]
- glucose homeostasis [IMP]
- glucose metabolic process [IMP]
- glucose transport [IMP]
- glycogen biosynthetic process [ISO]
- glycogen cell differentiation involved in embryonic placenta development [IMP]
- glycogen metabolic process [IMP]
- inflammatory response [IDA]
- insulin receptor signaling pathway [IMP, ISO]
- insulin-like growth factor receptor signaling pathway [ISO]
- intracellular signal transduction [ISO]
- labyrinthine layer blood vessel development [IMP]
- maternal placenta development [IMP]
- negative regulation of JNK cascade [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of autophagy [ISO]
- negative regulation of cell size [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of endopeptidase activity [ISO]
- negative regulation of fatty acid beta-oxidation [ISO]
- negative regulation of plasma membrane long-chain fatty acid transport [ISO]
- negative regulation of protein kinase activity [ISO]
- negative regulation of proteolysis [ISO]
- negative regulation of release of cytochrome c from mitochondria [IDA]
- osteoblast differentiation [IGI]
- peptidyl-serine phosphorylation [IDA, ISO]
- peripheral nervous system myelin maintenance [IMP]
- phosphorylation [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of cell growth [ISO]
- positive regulation of cellular protein metabolic process [ISO]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [ISO]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of lipid biosynthetic process [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of sodium ion transport [IDA, IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IGI]
- positive regulation of vasoconstriction [ISO]
- protein catabolic process [IDA]
- protein import into nucleus, translocation [ISO]
- protein kinase B signaling [IGI]
- protein phosphorylation [IDA, ISO]
- protein ubiquitination [IDA]
- regulation of cell migration [ISO]
- regulation of glycogen biosynthetic process [ISO]
- regulation of myelination [IMP]
- regulation of neuron projection development [IDA]
- regulation of protein localization [IDA, ISO]
- response to UV-A [ISO]
- response to fluid shear stress [ISO]
- response to food [IDA]
- response to hormone [IDA]
- signal transduction [ISO]
- striated muscle cell differentiation [IGI]
- translation [ISO]
Gene Ontology Molecular Function- 14-3-3 protein binding [ISO]
- ATP binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [ISO]
- protein kinase activity [IDA, ISO]
- protein kinase binding [IPI]
- protein phosphatase 2A binding [ISO]
- protein serine/threonine kinase activity [IDA, ISO]
- protein serine/threonine/tyrosine kinase activity [ISO]
- 14-3-3 protein binding [ISO]
- ATP binding [ISO]
- enzyme binding [ISO]
- identical protein binding [ISO]
- kinase activity [ISO]
- nitric-oxide synthase regulator activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- phosphatidylinositol-3,4-bisphosphate binding [ISO]
- protein binding [IPI]
- protein kinase C binding [ISO]
- protein kinase activity [IDA, ISO]
- protein kinase binding [IPI]
- protein phosphatase 2A binding [ISO]
- protein serine/threonine kinase activity [IDA, ISO]
- protein serine/threonine/tyrosine kinase activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Dephosphorylation and inactivation of Akt/PKB is counteracted by protein kinase CK2 in HEK 293T cells.
Akt (PKB) is a critical kinase in cell-survival pathways. Its activity depends on the phosphorylation of Thr308 and Ser473, by PDK1 and mTORC2, respectively. We found that Akt can be further stimulated through phosphorylation of Ser129 by another kinase, CK2. Here we show that phosphorylation of Akt at Ser129 also facilitates its association with Hsp90 chaperone, thus preventing Thr308 dephosphorylation. ... [more]
Cell. Mol. Life Sci. Oct. 01, 2009; 66(20);3363-73 [Pubmed: 19662498]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AKT1 HSP90AA1 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | 2375980 |
Curated By
- BioGRID