BAIT
HRR25
KTI14, serine/threonine protein kinase HRR25, L000000810, YPL204W
Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta)
GO Process (8)
GO Function (2)
GO Component (9)
Gene Ontology Biological Process
- DNA repair [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation [IMP]
- mitotic nuclear division [IMP]
- protein phosphorylation [IDA, IMP]
- ribosomal large subunit biogenesis [IMP]
- ribosomal small subunit biogenesis [IMP]
- tRNA wobble uridine modification [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
VHS2
YIL135C
Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.
Casein kinase 1δ (CK1δ) family members associate with microtubule-organizing centers (MTOCs) from yeast to humans, but their mitotic roles and targets have yet to be identified. We show here that budding yeast CK1δ, Hrr25, is a γ-tubulin small complex (γTuSC) binding factor. Moreover, Hrr25's association with γTuSC depends on its kinase activity and its noncatalytic central domain. Loss of Hrr25 ... [more]
Mol. Biol. Cell Jul. 01, 2015; 26(13);2505-18 [Pubmed: 25971801]
Throughput
- Low Throughput
Curated By
- BioGRID