RSP5
Gene Ontology Biological Process
- cellular response to UV [IMP]
- chromatin assembly or disassembly [IMP]
- late endosome to vacuole transport via multivesicular body sorting pathway [IMP, IPI]
- mitochondrion organization [IGI, IMP]
- positive regulation of endocytosis [IMP]
- positive regulation of fatty acid biosynthetic process [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IPI]
- protein autoubiquitination [IGI]
- protein monoubiquitination [IDA, IGI, IMP]
- protein polyubiquitination [IDA, IMP]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of actin cytoskeleton organization [IGI]
- regulation of dolichol biosynthetic process [IGI, IMP]
- regulation of ergosterol biosynthetic process [IGI, IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of mRNA export from nucleus [IMP, IPI]
- regulation of multivesicular body size [IMP]
- regulation of nitrogen utilization [IGI]
- regulation of phosphate metabolic process [IGI]
- regulation of protein localization [IMP, IPI]
- regulation of rRNA processing [IMP]
- regulation of ribosomal large subunit export from nucleus [IMP]
- regulation of tRNA export from nucleus [IMP]
- regulation of tRNA processing [IMP]
- regulation of ubiquinone biosynthetic process [IGI, IMP]
- response to drug [IMP, IPI]
- ribophagy [IGI]
- ubiquitin-dependent endocytosis [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DIA1
Gene Ontology Biological Process
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.
Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the discovery of substrates for E3 ligases on a global scale. ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- High Throughput: A ubiquitination assay was applied to a protein microarray in order to identify substrates of the E3 enzyme Rsp5.
- Low Throughput: The in vitro reaction contained E1, Ubc4 [E2], and ubiquitin.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RSP5 DIA1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 1516752 | |
| DIA1 RSP5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2555 | BioGRID | 2063606 | |
| RSP5 DIA1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | High | - | BioGRID | 570785 | |
| RSP5 DIA1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | High | - | BioGRID | 238372 | |
| DIA1 RSP5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| DIA1 RSP5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID