EST1
Gene Ontology Biological Process
Gene Ontology Molecular Function
APC1
Gene Ontology Biological Process
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IMP]
- exit from mitosis [IMP]
- metaphase/anaphase transition of mitotic cell cycle [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [IMP]
- protein ubiquitination [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomics of yeast telomerase identified Cdc48-Npl4-Ufd1 and Ufd4 as regulators of Est1 and telomere length.
Almost 400 genes affect yeast telomere length, including Est1, which is critical for recruitment and activation of telomerase. Here we use mass spectrometry to identify novel telomerase regulators by their co-purification with the telomerase holoenzyme. In addition to all known subunits, over 100 proteins are telomerase associated, including all three subunits of the essential Cdc48-Npl4-Ufd1 complex as well as three ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
APC1 EST1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1652 | BioGRID | 2010163 |
Curated By
- BioGRID