BAIT

SKT5

CAL2, CHS4, CSD4, L000001911, L000000332, YBL061C
Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SHC1

L000001879, YER096W
Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Long-Chain Polyprenols Promote Spore Wall Formation inSaccharomyces cerevisiae.

Hoffmann R, Grabinska K, Guan Z, Sessa WC, Neiman AM

Dolichols are isoprenoid lipids of varying length that act as sugar carriers in glycosylation reactions in the endoplasmic reticulum. InSaccharomyces cerevisiae, there are twocis-prenyltransferases that synthesize polyprenol-an essential precursor to dolichol. These enzymes are heterodimers composed of Nus1 and either Rer2 or Srt1. Rer2-Nus1 and Srt1-Nus1 can both generate dolichol in vegetative cells, butsrt1∆ cells grow normally whilerer2∆ grows very ... [more]

Genetics Dec. 01, 2017; 207(4);1371-1386 [Pubmed: 28978675]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: spore wall formation (APO:0000043)

Additional Notes

  • spore walls were normal in either single mutant while both the chitosan and dityrosine layers were absent in the chs4/shc1 double mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SHC1 SKT5
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
154266
SKT5 SHC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1477BioGRID
2078354

Curated By

  • BioGRID