BAIT

ADA2

SWI8, chromatin-binding transcription regulator ADA2, L000000029, YDR448W
Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
Saccharomyces cerevisiae (S288c)
PREY

HOG1

SSK3, mitogen-activated protein kinase HOG1, L000000797, YLR113W
Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII in osmotic shock; activates CDC28 by stimulating antisense RNA transcription; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; with Mrc1p defines novel S-phase checkpoint that prevent conflicts between DNA replication and transcription; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Selective requirement for SAGA in Hog1-mediated gene expression depending on the severity of the external osmostress conditions.

Zapater M, Sohrmann M, Peter M, Posas F, de Nadal E

Regulation of gene expression by the Hog1 stress-activated protein kinase is essential for proper cell adaptation to osmostress. Hog1 coordinates an extensive transcriptional program through the modulation of transcription. To identify systematically novel components of the transcriptional machinery required for osmostress-mediated gene expression, we performed an exhaustive genome-wide genetic screening, searching for mutations that render cells osmosensitive at high osmolarity ... [more]

Mol. Cell. Biol. Jun. 01, 2007; 27(11);3900-10 [Pubmed: 17403898]

Throughput

  • Low Throughput

Curated By

  • BioGRID