SPT3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HOG1
Gene Ontology Biological Process
- cellular response to heat [IMP]
- cellular response to osmotic stress [IMP]
- hyperosmotic response [IMP]
- osmosensory signaling pathway [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IMP]
- protein phosphorylation [IDA]
- regulation of nuclear cell cycle DNA replication [IDA]
- regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IDA]
- response to arsenic-containing substance [IGI, IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Selective requirement for SAGA in Hog1-mediated gene expression depending on the severity of the external osmostress conditions.
Regulation of gene expression by the Hog1 stress-activated protein kinase is essential for proper cell adaptation to osmostress. Hog1 coordinates an extensive transcriptional program through the modulation of transcription. To identify systematically novel components of the transcriptional machinery required for osmostress-mediated gene expression, we performed an exhaustive genome-wide genetic screening, searching for mutations that render cells osmosensitive at high osmolarity ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPT3 HOG1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.153 | BioGRID | 370321 | |
| HOG1 SPT3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.153 | BioGRID | 397788 | |
| HOG1 SPT3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.153 | BioGRID | 911444 | |
| SPT3 HOG1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.153 | BioGRID | 911445 |
Curated By
- BioGRID