BAIT

ERBB4

ALS19, HER4, p180erbB4
erb-b2 receptor tyrosine kinase 4
GO Process (34)
GO Function (6)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens
PREY

PIK3R2

MPPH, MPPH1, P85B, p85, p85-BETA
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Phosphotyrosine interactome of the ErbB-receptor kinase family.

Schulze WX, Deng L, Mann M

Interactions between short modified peptide motifs and modular protein domains are central events in cell signal-transduction. We determined interaction partners to all cytosolic tyrosine residues of the four members of the ErbB-receptor family in an unbiased fashion by quantitative proteomics using pull-down experiments with pairs of phosphorylated and nonphosphorylated synthetic peptides. Each receptor had characteristic preferences for interacting proteins and ... [more]

Mol. Syst. Biol. May. 27, 2006; 1(0);2005.0008 [Pubmed: 16729043]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERBB4 PIK3R2
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

High0.71BioGRID
2847077

Curated By

  • BioGRID