BAIT
BCKDK
BCKDKD, BDK
branched chain ketoacid dehydrogenase kinase
GO Process (4)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CAB39
MO25, CGI-66
calcium binding protein 39
GO Process (12)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- cell cycle arrest [TAS]
- cellular hypotonic response [IC]
- insulin receptor signaling pathway [TAS]
- intracellular signal transduction [IDA]
- negative regulation of potassium ion transmembrane transport [IC]
- negative regulation of potassium ion transmembrane transporter activity [IC]
- negative regulation of rubidium ion transmembrane transporter activity [IC]
- negative regulation of rubidium ion transport [IC]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of protein serine/threonine kinase activity [IDA]
- signal transduction by phosphorylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.503 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID