BAIT
ACTR3B
ARP11, ARP3BETA
ARP3 actin-related protein 3 homolog B (yeast)
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
TWF2
A6RP, A6r, PTK9L, MSTP011
twinfilin actin-binding protein 2
GO Process (10)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- barbed-end actin filament capping [ISS]
- cellular response to growth factor stimulus [IMP]
- cellular response to retinoic acid [IMP]
- negative regulation of actin filament polymerization [ISS]
- positive regulation of axon extension [IMP]
- positive regulation of lamellipodium assembly [IMP]
- positive regulation of neuron projection development [IMP]
- regulation of actin cytoskeleton organization [IMP]
- regulation of microvillus length [IC]
- sequestering of actin monomers [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.357 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID