BAIT
NOS1
IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS
nitric oxide synthase 1 (neuronal)
GO Process (30)
GO Function (14)
GO Component (11)
Gene Ontology Biological Process
- arginine catabolic process [IC]
- blood coagulation [TAS]
- cellular response to growth factor stimulus [ISS]
- exogenous drug catabolic process [ISS]
- interaction with host [TAS]
- multicellular organismal response to stress [IMP]
- myoblast fusion [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of calcium ion transport [ISS]
- negative regulation of calcium ion transport into cytosol [TAS]
- negative regulation of hydrolase activity [ISS]
- negative regulation of potassium ion transport [ISS]
- negative regulation of serotonin uptake [ISS]
- neurotransmitter biosynthetic process [TAS]
- nitric oxide biosynthetic process [IBA, ISS]
- nitric oxide mediated signal transduction [IBA]
- peptidyl-cysteine S-nitrosylation [ISS]
- phagosome maturation [TAS]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of histone acetylation [ISS]
- positive regulation of sodium ion transmembrane transport [ISS]
- positive regulation of the force of heart contraction [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of vasodilation [IDA, IMP]
- regulation of cardiac muscle contraction [TAS]
- regulation of sodium ion transport [ISS]
- response to heat [IDA]
- response to hypoxia [IEP]
- striated muscle contraction [ISS]
Gene Ontology Molecular Function- FMN binding [ISS]
- NADP binding [ISS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [TAS]
- cadmium ion binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- ion channel binding [ISS]
- nitric-oxide synthase activity [IBA, ISS]
- oxidoreductase activity [IBA]
- protein binding [IPI]
- scaffold protein binding [ISS]
- sodium channel regulator activity [ISS]
- tetrahydrobiopterin binding [NAS]
- FMN binding [ISS]
- NADP binding [ISS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [TAS]
- cadmium ion binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- ion channel binding [ISS]
- nitric-oxide synthase activity [IBA, ISS]
- oxidoreductase activity [IBA]
- protein binding [IPI]
- scaffold protein binding [ISS]
- sodium channel regulator activity [ISS]
- tetrahydrobiopterin binding [NAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
CTBP1
50-kDaBFA-inducedADP-ribosylatedsubstrate, BARS-50, Bars, CtBP3/BARS
C-terminal binding protein 1
GO Process (9)
GO Function (10)
GO Component (5)
Gene Ontology Biological Process
- Golgi organization [IMP]
- membrane fusion [TAS]
- negative regulation of histone H4 acetylation [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of histone deacetylation [ISO]
- regulation of cell cycle [ISO]
- white fat cell differentiation [ISO, ISS]
Gene Ontology Molecular Function- NAD binding [IDA]
- RNA polymerase II transcription corepressor activity [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [IPI]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription corepressor activity [TAS]
- transcription factor binding [ISO]
- transferase activity, transferring acyl groups [TAS]
- NAD binding [IDA]
- RNA polymerase II transcription corepressor activity [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [IPI]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription corepressor activity [TAS]
- transcription factor binding [ISO]
- transferase activity, transferring acyl groups [TAS]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Binding of neuronal nitric-oxide synthase (nNOS) to carboxyl-terminal-binding protein (CtBP) changes the localization of CtBP from the nucleus to the cytosol: a novel function for targeting by the PDZ domain of nNOS.
Recent work suggests a role for PDZ domains in the targeting of binding partners to specific sites in the cell. To identify whether the PDZ domain of neuronal nitric-oxide synthase (nNOS) can play such a role, we performed affinity chromatography of brain extract with the nNOS PDZ domain. We identified the carboxyl-terminal-binding protein (CtBP), a phosphoprotein first identified as a ... [more]
J. Biol. Chem. Dec. 21, 2001; 276(51);48262-8 [Pubmed: 11590170]
Throughput
- Low Throughput
Additional Notes
- Affinity chromatography of brain extract with the nNOS PDZ domain
Curated By
- BioGRID