PREY

AR

AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, RP11-383C12.1
androgen receptor
GO Process (23)
GO Function (16)
GO Component (5)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interactions between activating signal cointegrator-2 and the tumor suppressor retinoblastoma in androgen receptor transactivation.

Goo YH, Na SY, Zhang H, Xu J, Hong S, Cheong J, Lee SK, Lee JW

Activating signal cointegrator-2 (ASC-2), a cancer-amplified transcription coactivator of nuclear receptors and numerous other transcription factors, was previously shown to contain two LXXLL motifs, each of which interacts with a distinct set of nuclear receptors. In this work, we showed that ASC-2 has an indirect, separate binding site for androgen receptor (AR). Interestingly, this region overlapped with the direct interaction ... [more]

J. Biol. Chem. Feb. 20, 2004; 279(8);7131-5 [Pubmed: 14645241]

Throughput

  • Low Throughput

Additional Notes

  • AR interacted with ASC2-2b that consists of the ASC-2 residues 622-849 in a testosterone-independent manner; indirect

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AR NCOA6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
AR NCOA6
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High1BioGRID
3324356

Curated By

  • BioGRID