BAIT
EAF3
YPR023C
Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
GO Process (8)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- DNA repair [IDA]
- histone acetylation [IDA]
- histone deacetylation [IMP]
- negative regulation of antisense RNA transcription [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- regulation of DNA-dependent DNA replication initiation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
KIN28
TFIIH complex serine/threonine-protein kinase subunit KIN28, L000000905, YDL108W
Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia
GO Process (8)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [IMP]
- phosphorylation of RNA polymerase II C-terminal domain [IDA, IMP]
- phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP, IPI]
- phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein phosphorylation [IDA]
- transcription from RNA polymerase I promoter [IMP]
- transcription from RNA polymerase II promoter [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.221685 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Digenic interaction: Query allele name: eaf3-delta+ho-delta; Array allele name: kin28-ts (GI score = -0.221685, p-value = 0.0130; Digenic)
- Digenic interactions in this Synthetic genetic array (SGA) analysis were considered to be significant when epsilon > 0.08 and p < 0.05 (positive genetic interaction) and when epsilon < -0.08 and p < 0.05 (negative genetic interaction).
Curated By
- BioGRID