BAIT

ATM

AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
ATM serine/threonine kinase
Homo sapiens
PREY

AP3B2

AI549966, AU042881, Naptb, [b]-NAP, beta-NAP, beta3B
adaptor-related protein complex 3, beta 2 subunit
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ATM binds to beta-adaptin in cytoplasmic vesicles.

Lim DS, Kirsch DG, Canman CE, Ahn JH, Ziv Y, Newman LS, Darnell RB, Shiloh Y, Kastan MB

Inherited mutations in the ATM gene lead to a complex clinical phenotype characterized by neuronal degeneration, oculocutaneous telangiectasias, immune dysfunction, and cancer predisposition. Using the yeast two-hybrid system, we demonstrate that ataxia telangiectasia mutated (ATM) binds to beta-adaptin, one of the components of the AP-2 adaptor complex, which is involved in clathrin-mediated endocytosis of receptors. The interaction between ATM and ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 18, 1998; 95(17);10146-51 [Pubmed: 9707615]

Throughput

  • Low Throughput

Additional Notes

  • DNA encoding FB2F (amino acids 811-1,283)

Curated By

  • BioGRID