BAIT
PWP1
L000001539, YLR196W
Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
GO Process (1)
GO Function (0)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY
RTG2
L000001784, YGL252C
Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
GO Process (7)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- maintenance of DNA trinucleotide repeats [IMP]
- mitochondria-nucleus signaling pathway [IMP]
- negative regulation of extrachromosomal rDNA circle accumulation involved in replicative cell aging [IMP]
- nitrogen catabolite regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- transcription factor import into nucleus [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.152289 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Trigenic interaction: Query allele name: PWP1-ph+gis2-delta; Array allele name: rtg2-delta (GI score = -0.152289, p-value = 0.00679; Novel)
- Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.
Curated By
- BioGRID