BAIT
ISR1
putative protein kinase ISR1, L000004210, YPR106W
Predicted protein kinase; overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
RTF1
CSL3, L000001782, YGL244W
Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
GO Process (19)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- global genome nucleotide-excision repair [IMP]
- mRNA 3'-end processing [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]
- regulation of chromatin silencing at telomere [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA, IMP]
- regulation of histone H2B ubiquitination [IMP]
- regulation of histone H3-K4 methylation [IMP]
- regulation of histone H3-K79 methylation [IMP]
- regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- regulation of transcription from RNA polymerase II promoter [IGI]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- snoRNA 3'-end processing [IMP]
- snoRNA transcription from an RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.156334 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Trigenic interaction: Query allele name: rim20-delta+isr1-delta; Array allele name: rtf1-delta (GI score = -0.156334, p-value = 0.00652; Novel)
- Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.
Curated By
- BioGRID