SMAD7
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- adherens junction assembly [IMP]
- artery morphogenesis [ISS]
- cellular protein complex localization [IDA]
- cellular response to transforming growth factor beta stimulus [IMP]
- gene expression [TAS]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell migration [TAS]
- negative regulation of epithelial to mesenchymal transition [IC, TAS]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, TAS]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of peptidyl-threonine phosphorylation [IDA]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription by competitive promoter binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA, TAS]
- negative regulation of ubiquitin-protein transferase activity [IDA]
- pathway-restricted SMAD protein phosphorylation [ISS]
- positive regulation of cell-cell adhesion [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- protein stabilization [IDA]
- regulation of activin receptor signaling pathway [IDA]
- regulation of cardiac muscle contraction [ISS]
- regulation of transforming growth factor beta receptor signaling pathway [IC]
- regulation of ventricular cardiac muscle cell membrane depolarization [IC]
- response to laminar fluid shear stress [IEP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ventricular cardiac muscle tissue morphogenesis [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- activin binding [IPI]
- beta-catenin binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- activin binding [IPI]
- beta-catenin binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
BMPR1A
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- endocardial cushion formation [ISS]
- immune response [IMP]
- positive regulation of SMAD protein import into nucleus [IDA]
- positive regulation of bone mineralization [IMP]
- positive regulation of osteoblast differentiation [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- protein phosphorylation [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- caveola [IMP]
- plasma membrane [IDA, TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Roles for the MH2 domain of Smad7 in the specific inhibition of transforming growth factor-beta superfamily signaling.
Signals by cytokines of the transforming growth factor-beta (TGF-beta) superfamily are negatively regulated by inhibitory Smads (I-Smads). Smad7 inhibits signaling by both TGF-beta and bone morphogenetic proteins (BMPs), whereas Smad6 inhibits TGF-beta signals less effectively. I-Smads have amino-terminal N domains and carboxyl-terminal Mad homology 2 (MH2) domains. The N domains are essential for specific inhibition of TGF-beta signaling by Smad7, ... [more]
Throughput
- Low Throughput
Additional Notes
- Identified four basic amino acid residues (Lys-312, Lys-316, Lys-401, and Arg-409) in the basic surface of the Smad7 MH2 domain that play important roles in interaction with type I receptors
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD7 BMPR1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9351 | BioGRID | 3293158 | |
| SMAD7 BMPR1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID