BAIT
HOG1
SSK3, mitogen-activated protein kinase HOG1, L000000797, YLR113W
Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII in osmotic shock; activates CDC28 by stimulating antisense RNA transcription; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; with Mrc1p defines novel S-phase checkpoint that prevent conflicts between DNA replication and transcription; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
GO Process (10)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- cellular response to heat [IMP]
- cellular response to osmotic stress [IMP]
- hyperosmotic response [IMP]
- osmosensory signaling pathway [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IMP]
- protein phosphorylation [IDA]
- regulation of nuclear cell cycle DNA replication [IDA]
- regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IDA]
- response to arsenic-containing substance [IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
NHP6A
L000001244, L000001245, YPR052C
High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
GO Process (6)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- DNA replication-independent nucleosome organization [IDA]
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- RNA polymerase III transcriptional preinitiation complex assembly [IDA, IGI]
- chromatin remodeling [IGI]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter [IDA]
- mismatch repair [IC]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Network inference reveals novel connections in pathways regulating growth and defense in the yeast salt response.
Cells respond to stressful conditions by coordinating a complex, multi-faceted response that spans many levels of physiology. Much of the response is coordinated by changes in protein phosphorylation. Although the regulators of transcriptome changes during stress are well characterized in Saccharomyces cerevisiae, the upstream regulatory network controlling protein phosphorylation is less well dissected. Here, we developed a computational approach to ... [more]
PLoS Comput. Biol. Dec. 01, 2017; 13(5);e1006088 [Pubmed: 29738528]
Throughput
- High Throughput
Curated By
- BioGRID