BAIT
SSK1
mitogen-activated protein kinase kinase kinase SSK1, L000002084, YLR006C
Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
GO Process (4)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
PTP2
tyrosine protein phosphatase PTP2, L000001526, YOR208W
Nuclear phosphotyrosine-specific phosphatase involved in osmosensing; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; with Msg5p co-regulates the calcium signaling pathway
GO Process (5)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Activation of the Hog1 MAPK by the Ssk2/Ssk22 MAP3Ks, in the absence of the osmosensors, is not sufficient to trigger osmostress adaptation in Saccharomyces cerevisiae.
Yeast cells respond to hyperosmotic stress by activating the high-osmolarity glycerol (HOG) pathway, which consists of two branches, Hkr1/Msb2-Sho1 and Sln1, which trigger phosphorylation and nuclear internalization of the Hog1 mitogen-activated protein kinase. In the nucleus, Hog1 regulates gene transcription and cell cycle progression, which allows the cell to respond and adapt to hyperosmotic conditions. This study demonstrates that the ... [more]
FEBS J. Mar. 01, 2018; 285(6);1079-1096 [Pubmed: 29341399]
Throughput
- Low Throughput
Ontology Terms
- phenotype: protein/peptide modification (APO:0000131)
- phenotype: osmotic stress resistance (APO:0000082)
Additional Notes
- deletion of ptp2 increases growth in osmotic stress in the ssk1/ste11 double mutant
- genetic complex
Related interactions
Curated By
- BioGRID