An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Functional interaction of monoubiquitinated FANCD2 and BRCA2/FANCD1 in chromatin.

Wang X, Andreassen PR, D'Andrea AD

Fanconi anemia (FA) is an autosomal recessive cancer susceptibility syndrome with at least 11 complementation groups (A, B, C, D1, D2, E, F, G, I, J, and L), and eight FA genes have been cloned. The FANCD1 gene is identical to the breast cancer susceptibility gene, BRCA2. The FA proteins cooperate in a common pathway, but the function of BRCA2/FANCD1 ... [more]

Mol. Cell. Biol. Jul. 01, 2004; 24(13);5850-62 [Pubmed: 15199141]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRCA2 FANCE	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Mengwasser KE (2019)	Low	-	BioGRID	3656558

Curated By

BioGRID

Interaction Summary

BRCA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

H3 histone acetyltransferase activity [IDA]H4 histone acetyltransferase activity [IDA]gamma-tubulin binding [IPI]histone acetyltransferase activity [IDA]protease binding [IPI]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

FANCE