BAIT
CDC16
ANAPC6, APC6, CUT9, RP11-569D9.4
cell division cycle 16
GO Process (9)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- cell proliferation [TAS]
- mitotic cell cycle [TAS]
- mitotic spindle assembly checkpoint [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein K11-linked ubiquitination [IDA]
- regulation of mitosis [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NEMF
NY-CO-1, SDCCAG1
nuclear export mediator factor
GO Process (1)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Cell Jul. 17, 2018; (); [Pubmed: 30033366]
Quantitative Score
- -3.759971515 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: k-562 cell (BTO:0000664)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- K562 cell line Replicate Average GI score = -3.759971515
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Curated By
- BioGRID