BAIT
PPP2R1A
PP2A-Aalpha, PP2AAALPHA, PR65A
protein phosphatase 2, regulatory subunit A, alpha
GO Process (25)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- RNA metabolic process [TAS]
- RNA splicing [NAS]
- apoptotic process [TAS]
- ceramide metabolic process [NAS]
- chromosome segregation [IDA]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- inactivation of MAPK activity [NAS]
- mRNA metabolic process [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope reassembly [TAS]
- negative regulation of cell growth [NAS]
- negative regulation of tyrosine phosphorylation of Stat3 protein [NAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- protein complex assembly [TAS]
- protein dephosphorylation [TAS]
- regulation of DNA replication [NAS]
- regulation of Wnt signaling pathway [NAS]
- regulation of cell adhesion [NAS]
- regulation of cell differentiation [NAS]
- regulation of growth [NAS]
- regulation of transcription, DNA-templated [NAS]
- response to organic substance [NAS]
- second-messenger-mediated signaling [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TAF1
BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
GO Process (15)
GO Function (10)
GO Component (3)
Gene Ontology Biological Process
- DNA-templated transcription, initiation [ISS]
- RNA polymerase II transcriptional preinitiation complex assembly [ISS]
- cellular response to DNA damage stimulus [IC]
- gene expression [TAS]
- histone acetylation [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI]
- positive regulation of transcription initiation from RNA polymerase II promoter [ISS]
- protein autophosphorylation [IDA, TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
- TBP-class protein binding [IPI]
- histone acetyltransferase activity [IDA]
- lysine-acetylated histone binding [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- protein serine/threonine kinase activity [IDA]
- sequence-specific DNA binding [ISS]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Mapping the Genetic Landscape of Human Cells.
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. We developed a CRISPR interference platform for large-scale quantitative mapping of human GIs. We systematically perturbed 222,784 ... [more]
Cell Jul. 17, 2018; (); [Pubmed: 30033366]
Quantitative Score
- -3.091803642 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- cell type: k-562 cell (BTO:0000664)
Additional Notes
- CRISPR GI screen
- Cell Line: K562 EFO:0002067/Jurkat EFO:0002796
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative/positive genetic interaction
- Interactions in this CRISPR interference (CRISPRi) analysis were considered to be significant when GI <= -3 (negative genetic interaction) or GI >= 3 (positive genetic interaction).
- K562 cell line Replicate Average GI score = -3.091803642
- Library: CRISPRi v1
- Significance Threshold: (positive genetic interaction) 3
Curated By
- BioGRID