MAPK3
Gene Ontology Biological Process
- BMP signaling pathway [IMP]
- DNA damage induced protein phosphorylation [IDA]
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- MAPK cascade [NAS, TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- Ras protein signal transduction [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- caveolin-mediated endocytosis [TAS]
- cellular response to mechanical stimulus [IEP]
- cytokine-mediated signaling pathway [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- interleukin-1-mediated signaling pathway [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-tyrosine autophosphorylation [IDA]
- phosphorylation [IDA]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of histone acetylation [IMP]
- positive regulation of histone phosphorylation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein phosphorylation [IDA]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of sequence-specific DNA binding transcription factor activity [TAS]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [IDA]
- small GTPase mediated signal transduction [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KLF4
Gene Ontology Biological Process
- canonical Wnt signaling pathway [IMP]
- cell differentiation [IMP]
- cellular response to growth factor stimulus [ISO]
- cellular response to laminar fluid shear stress [ISO]
- epidermal cell differentiation [IMP]
- epidermis morphogenesis [IMP]
- fat cell differentiation [IMP]
- negative regulation of ERK1 and ERK2 cascade [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of cell migration involved in sprouting angiogenesis [ISO]
- negative regulation of cell proliferation [IDA]
- negative regulation of chemokine (C-X-C motif) ligand 2 production [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of heterotypic cell-cell adhesion [ISO]
- negative regulation of interleukin-8 biosynthetic process [ISO]
- negative regulation of muscle hyperplasia [ISO]
- negative regulation of phosphatidylinositol 3-kinase signaling [ISO]
- negative regulation of protein kinase B signaling [ISO]
- negative regulation of response to cytokine stimulus [ISO]
- negative regulation of smooth muscle cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS, NAS]
- positive regulation of cellular protein metabolic process [ISO]
- positive regulation of hemoglobin biosynthetic process [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of protein metabolic process [ISO]
- positive regulation of telomerase activity [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- post-embryonic camera-type eye development [IMP]
- post-embryonic hemopoiesis [ISO]
- regulation of cell differentiation [IDA]
- regulation of cell proliferation [IGI]
- regulation of phosphatidylinositol 3-kinase activity [ISO]
- regulation of transcription from RNA polymerase II promoter [IDA]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- stem cell maintenance [IDA]
- transcription from RNA polymerase II promoter [IDA, ISO]
Gene Ontology Molecular Function- DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II transcription factor binding [IPI, ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [IPI]
- core promoter binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- double-stranded DNA binding [ISO]
- histone deacetylase binding [ISO]
- phosphatidylinositol 3-kinase regulator activity [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor recruiting transcription factor activity [IDA]
- transcription cofactor binding [ISO]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA, ISO]
- DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II transcription factor binding [IPI, ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [IPI]
- core promoter binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- double-stranded DNA binding [ISO]
- histone deacetylase binding [ISO]
- phosphatidylinositol 3-kinase regulator activity [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor recruiting transcription factor activity [IDA]
- transcription cofactor binding [ISO]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA, ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
ERK1 and ERK2 regulate embryonic stem cell self-renewal through phosphorylation of Klf4.
Understanding and controlling the mechanism by which stem cells balance self-renewal versus differentiation is of great importance for stem cell therapeutics. Klf4 promotes the self-renewal of embryonic stem cells, but the precise mechanism regulating this role of Klf4 is unclear. We found that ERK1 or ERK2 binds the activation domain of Klf4 and directly phosphorylates Klf4 at Ser123. This phosphorylation ... [more]
Throughput
- Low Throughput
Additional Notes
- Source of MAPK3 not clear
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
KLF4 MAPK3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 2461115 | |
MAPK3 KLF4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2461112 | |
KLF4 MAPK3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 2461109 |
Curated By
- BioGRID