Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease.

Teixeira FR, Randle SJ, Patel SP, Mevissen TE, Zenkeviciute G, Koide T, Komander D, Laman H

Fbxo7 is a clinically relevant F-box protein, associated with both cancer and Parkinson's disease (PD). Additionally, SNPs within FBXO7 are correlated with alterations in red blood cell parameters. Point mutations within FBXO7 map within specific functional domains, including near its F-box domain and its substrate recruiting domains, suggesting that deficiencies in SCF(Fbxo7/PARK15) ubiquitin ligase activity are mechanistically linked to early-onset ... [more]

Biochem. J. Oct. 15, 2016; 473(20);3563-3580 [Pubmed: 27503909]

Throughput

  • High Throughput

Additional Notes

  • hits identified in IP of both wt and delta-Fbox mutant of Fbxo7

Curated By

  • BioGRID