BAIT

CUT1

ptr4, SPCC5E4.04
separase/separin
GO Process (3)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

NUC2

SPAC1851.01, apc3, SPAC17C9.01c
anaphase-promoting complex TPR repeat subunit Apc3
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T, Yuasa T, Hayashi T, Obara T, Kimata Y, Yanagida M

BACKGROUND: The fission yeast Schizosaccharomyces pombe separase/Cut1 and securin/Cut2 are required for anaphase-specific activation of proteolysis that leads to proper sister chromatid separation. We intended to identify ts (temperature sensitive) strains whose growth was inhibited by multicopy plasmid pCUT1 or pCUT2 at the permissive temperature. RESULTS: After a one-by-one transformation of 1015 randomly isolated ts strains, 18 transformants that retarded ... [more]

Genes Cells Apr. 01, 2003; 8(4);341-55 [Pubmed: 12653962]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUT1 NUC2
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
246404

Curated By

  • BioGRID