A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast.

Hermand D, Westerling T, Pihlak A, Thuret JY, Vallenius T, Tiainen M, Vandenhaute J, Cottarel G, Mann C, Maekelae TP

Activating phosphorylation of cyclin-dependent kinases (Cdks) is mediated by at least two structurally distinct types of Cdk-activating kinases (Caks): the trimeric Cdk7-cyclin H-Mat1 complex in metazoans and the single-subunit Cak1 in budding yeast. Fission yeast has both Cak types: Mcs6 is a Cdk7 ortholog and Csk1 a single-subunit kinase. Both phosphorylate Cdks in vitro and rescue a thermosensitive budding yeast ... [more]

EMBO J. Jan. 15, 2001; 20(1);82-90 [Pubmed: 11226158]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CSK1 MCS6	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Hermand D (1998)	Low	-	BioGRID	250547
CSK1 MCS6	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Lee KM (1999)	Low	-	BioGRID	247376
CSK1 MCS6	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Saiz JE (2002)	Low	-	BioGRID	246714
CSK1 MCS6	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Hermand D (1998)	Low	-	BioGRID	246333
MCS6 CSK1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Hermand D (1998)	Low	-	BioGRID	246334
CSK1 MCS6	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Hermand D (2001)	Low	-	BioGRID	246335

Curated By

BioGRID

Interaction Summary

MCS6

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II carboxy-terminal domain kinase activity [IDA]cyclin-dependent protein kinase activating kinase activity [IDA, IGI]

Gene Ontology Cellular Component

CSK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cyclin-dependent protein kinase activating kinase activity [IGI]cyclin-dependent protein serine/threonine kinase regulator activity [IMP]protein kinase activator activity [IDA]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]