A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast.

Cullen CF, May KM, Hagan IM, Glover DM, Ohkura H

We describe a general genetic method to identify genes encoding proteins that functionally interact with and/or are good candidates for downstream targets of a particular gene product. The screen identifies mutants whose growth depends on high levels of expression of that gene. We apply this to the plo1(+) gene that encodes a fission yeast homologue of the polo-like kinases. plo1(+) ... [more]

Genetics Aug. 01, 2000; 155(4);1521-34 [Pubmed: 10924454]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: heat sensitivity (APO:0000147)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPG1 CDC7	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Chew TG (2008)	Low	-	BioGRID	-
SPG1 CDC7	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mehta S (2006)	Low	-	BioGRID	-
CDC7 SPG1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mehta S (2006)	Low	-	BioGRID	-
SPG1 CDC7	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Schmidt S (1997)	Low	-	BioGRID	-
CDC7 SPG1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sohrmann M (1998)	Low	-	BioGRID	-
SPG1 CDC7	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Balasubramanian MK (1998)	Low	-	PomBase	-
CDC7 SPG1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Schmidt S (1997)	Low	-	BioGRID	882967
SPG1 CDC7	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Schmidt S (1997)	Low	-	BioGRID	246830
SPG1 CDC7	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Mehta S (2006)	Low	-	BioGRID	-
CDC7 SPG1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Mehta S (2006)	Low	-	BioGRID	-
CDC7 SPG1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Balasubramanian MK (1998)	Low	-	BioGRID	246398
SPG1 CDC7	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Schmidt S (1997)	Low	-	PomBase	882974
SPG1 CDC7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Mehta S (2006)	Low	-	BioGRID	-
SPG1 CDC7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Schmidt S (1997)	Low	-	BioGRID	-
SPG1 CDC7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vo TV (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC7

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein serine/threonine kinase activity [IDA, IMP]

Gene Ontology Cellular Component

SPG1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [TAS]protein binding [IPI]