Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Phosphorylation of XIAP by CDK1-cyclin-B1 controls mitotic cell death.

Hou Y, Allan LA, Clarke PR

Regulation of cell death is crucial for the response of cancer cells to drug treatments that cause arrest in mitosis, and is likely to be important for protection against chromosome instability in normal cells. Prolonged mitotic arrest can result in cell death by activation of caspases and the induction of apoptosis. Here, we show that X-linked inhibitor of apoptosis (XIAP) ... [more]

J. Cell. Sci. Dec. 15, 2016; 130(2);502-511 [Pubmed: 27927753]

Throughput

  • Low Throughput

Additional Notes

  • the ability of CDK1/Cyclin B1 (CCNB1) to bind XIAP is deduced from the ability of CDK1/Cyclin B1 to phosphorylate XIAP in vitro

Curated By

  • BioGRID