BAIT

PPP1R3C

PPP1R5
protein phosphatase 1, regulatory subunit 3C
GO Process (5)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PPP1CB

HEL-S-80p, PP-1B, PP1B, PP1beta, PPP1CD
protein phosphatase 1, catalytic subunit, beta isozyme
Alzheimer's Disease Project
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.

Yadav L, Tamene F, Goeoes H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M

Coordinated activities of protein kinases and phosphatases ensure phosphorylation homeostasis, which, when perturbed, can instigate diseases, including cancer. Yet, in contrast to kinases, much less is known about protein phosphatase functions and their interactions and complexes. Here, we used quantitative affinity proteomics to assay protein-protein interactions for 54 phosphatases distributed across the three major protein phosphatase families, with additional analysis ... [more]

Cell Syst Apr. 26, 2017; 4(4);430-444.e5 [Pubmed: 28330616]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPP1R3C PPP1CB
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2717942
PPP1CB PPP1R3C
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID