An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in Î²-Catenin-Active Cancers.

Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe J, Lage K, Boehm JS, Hahn WC

Genome-scale expression studies and comprehensive loss-of-function genetic screens have focused almost exclusively on the highest confidence candidate genes. Here, we describe a strategy for characterizing the lower confidence candidates identified by such approaches. We interrogated 177 genes that we classified as essential for the proliferation of cancer cells exhibiting constitutive Î²-catenin activity and integrated data for each of the candidates, ... [more]

Cell Syst Dec. 28, 2015; 3(3);302-316.e4 [Pubmed: 27684187]

Throughput

High Throughput

Additional Notes

High-credibility protein interactions were identified using draft-PPI and a method for protein interaction credibility scoring (ICS).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KIF5B COPE	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Kristensen AR (2012)	High	-	BioGRID	920720

Curated By

BioGRID

Interaction Summary

COPE

Gene Ontology Biological Process

Gene Ontology Cellular Component

KIF5B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IBA]microtubule binding [ISS]microtubule motor activity [ISS]plus-end-directed microtubule motor activity [IBA]protein binding [IPI]