Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.

Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP

Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]

Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]

Throughput

High Throughput

Additional Notes

High confidence rec-Y2H protein interactions.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CASK RBM10	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yang JS (2018)	High	-	BioGRID	2474280

Curated By

BioGRID

Interaction Summary

RBM10

Gene Ontology Molecular Function

identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

CASK

Gene Ontology Biological Process

Gene Ontology Molecular Function

guanylate kinase activity [TAS]protein binding [IPI]