BAIT
CASK
CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8, RP11-540L11.1
calcium/calmodulin-dependent serine protein kinase (MAGUK family)
GO Process (7)
GO Function (2)
GO Component (11)
Gene Ontology Biological Process
- cell adhesion [TAS]
- extracellular matrix organization [TAS]
- negative regulation of cell-matrix adhesion [IMP]
- negative regulation of cellular response to growth factor stimulus [IMP]
- negative regulation of keratinocyte proliferation [IMP]
- negative regulation of wound healing [IMP]
- nucleotide phosphorylation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KIF2A
CDCBM3, HK2, KIF2
kinesin heavy chain member 2A
GO Process (9)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- blood coagulation [TAS]
- metabolic process [IBA, TAS]
- microtubule depolymerization [IBA]
- microtubule-based movement [IBA, TAS]
- mitotic cell cycle [TAS]
- mitotic spindle organization [IDA]
- spindle assembly involved in mitosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of detecting interactions within protein libraries or between protein libraries and RNA fragment pools. rec-YnH combines batch cloning and transformation with intracellular ... [more]
Nat Commun Sep. 14, 2018; 9(1);3747 [Pubmed: 30217970]
Throughput
- High Throughput
Additional Notes
- High confidence rec-Y2H protein interactions.
Curated By
- BioGRID