BAIT

PSF2

bsh3, dre13, SPBC725.13c
GINS complex subunit Psf2
GO Process (3)
GO Function (0)
GO Component (4)
Schizosaccharomyces pombe (972h)
PREY

RAD3

SPBC216.05
ATR checkpoint kinase Rad3
GO Process (3)
GO Function (2)
GO Component (3)
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+.

Gomez EB, Angeles VT, Forsburg SL

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen ... [more]

Genetics Jan. 01, 2005; 169(1);77-89 [Pubmed: 15466421]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID