BAIT

DEF1

VID31, DNA damage-responsive RNA polymerase-degradation factor DEF1, YKL054C
RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

TFB1

TFIIH/NER complex subunit TFB1, L000002284, YDR311W
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Def1 interacts with TFIIH and modulates RNA polymerase II transcription.

Damodaren N, Van Eeuwen T, Zamel J, Lin-Shiao E, Kalisman N, Murakami K

The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans, Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2017; 114(50);13230-13235 [Pubmed: 29180430]

Throughput

  • High Throughput

Additional Notes

  • The crude eluate of tap-tagged Def1-TFIIH was subjected to MS-based semiquantitative proteomics analysis and cross-linking followed by mass spectrometry (MS). The MS analysis identified ~170 proteins with two or more unique peptides.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DEF1 TFB1
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
2484185
DEF1 TFB1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1535BioGRID
2053556

Curated By

  • BioGRID