DEF1
Gene Ontology Biological Process
RPN11
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Def1 interacts with TFIIH and modulates RNA polymerase II transcription.
The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans, Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response ... [more]
Throughput
- High Throughput
Additional Notes
- The crude eluate of tap-tagged Def1-TFIIH was subjected to MS-based semiquantitative proteomics analysis and cross-linking followed by mass spectrometry (MS). The MS analysis identified ~170 proteins with two or more unique peptides.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
DEF1 RPN11 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2555 | BioGRID | 2053560 |
Curated By
- BioGRID