BAIT

DEF1

VID31, DNA damage-responsive RNA polymerase-degradation factor DEF1, YKL054C
RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

SOH1

MED31, L000001982, YGL127C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
GO Process (5)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Def1 interacts with TFIIH and modulates RNA polymerase II transcription.

Damodaren N, Van Eeuwen T, Zamel J, Lin-Shiao E, Kalisman N, Murakami K

The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans, Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2017; 114(50);13230-13235 [Pubmed: 29180430]

Throughput

  • High Throughput

Additional Notes

  • The crude eluate of tap-tagged Def1-TFIIH was subjected to MS-based semiquantitative proteomics analysis and cross-linking followed by mass spectrometry (MS). The MS analysis identified ~170 proteins with two or more unique peptides.

Curated By

  • BioGRID