BAIT

DEF1

VID31, DNA damage-responsive RNA polymerase-degradation factor DEF1, YKL054C
RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

SKI6

ECM20, RRP41, L000003895, L000004426, YGR195W
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Def1 interacts with TFIIH and modulates RNA polymerase II transcription.

Damodaren N, Van Eeuwen T, Zamel J, Lin-Shiao E, Kalisman N, Murakami K

The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans, Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 12, 2017; 114(50);13230-13235 [Pubmed: 29180430]

Throughput

  • High Throughput

Additional Notes

  • The crude eluate of tap-tagged Def1-TFIIH was subjected to MS-based semiquantitative proteomics analysis and cross-linking followed by mass spectrometry (MS). The MS analysis identified ~170 proteins with two or more unique peptides.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DEF1 SKI6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2841BioGRID
2053565

Curated By

  • BioGRID