BAIT
UBE2M
UBC-RS2, UBC12, hUbc12
ubiquitin-conjugating enzyme E2M
GO Process (4)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
HPRT1
HGPRT, HPRT
hypoxanthine phosphoribosyltransferase 1
GO Process (16)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- GMP catabolic process [IDA]
- GMP salvage [IBA]
- IMP metabolic process [IDA]
- IMP salvage [IBA]
- adenine salvage [IBA]
- guanine salvage [IDA]
- hypoxanthine metabolic process [IMP]
- hypoxanthine salvage [IDA]
- nucleobase-containing small molecule metabolic process [TAS]
- positive regulation of dopamine metabolic process [IMP]
- protein homotetramerization [IDA, IPI]
- purine nucleobase metabolic process [TAS]
- purine nucleotide biosynthetic process [IMP]
- purine ribonucleoside salvage [IMP]
- purine-containing compound salvage [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
N-terminal acetylation is an abundant modification influencing protein functions. Because ∼80% of mammalian cytosolic proteins are N-terminally acetylated, this modification is potentially an untapped target for chemical control of their functions. Structural studies have revealed that, like lysine acetylation, N-terminal acetylation converts a positively charged amine into a hydrophobic handle that mediates protein interactions; hence, this modification may be a ... [more]
Nat. Chem. Biol. Aug. 01, 2017; 13(8);850-857 [Pubmed: 28581483]
Throughput
- High Throughput
Curated By
- BioGRID