BAIT
UBE2M
UBC-RS2, UBC12, hUbc12
ubiquitin-conjugating enzyme E2M
GO Process (4)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
SNRPE
B-raf, HYPT11, SME, Sm-E
small nuclear ribonucleoprotein polypeptide E
GO Process (12)
GO Function (1)
GO Component (18)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- hair cycle [IMP]
- histone mRNA metabolic process [TAS]
- mRNA 3'-end processing [TAS]
- mRNA splicing, via spliceosome [IC, TAS]
- ncRNA metabolic process [TAS]
- spliceosomal complex assembly [NAS]
- spliceosomal snRNP assembly [IDA, TAS]
- termination of RNA polymerase II transcription [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SMN-Sm protein complex [IDA]
- U1 snRNP [IDA]
- U12-type spliceosomal complex [IDA]
- U2 snRNP [IBA]
- U4 snRNP [IDA]
- U4/U6 x U5 tri-snRNP complex [IBA]
- U5 snRNP [IBA]
- U7 snRNP [IDA]
- catalytic step 2 spliceosome [IDA]
- cytosol [IDA, TAS]
- extracellular vesicular exosome [IDA]
- methylosome [IDA]
- nucleoplasm [TAS]
- nucleus [IDA]
- pICln-Sm protein complex [IDA]
- precatalytic spliceosome [IBA]
- small nuclear ribonucleoprotein complex [NAS]
- spliceosomal complex [NAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
N-terminal acetylation is an abundant modification influencing protein functions. Because ∼80% of mammalian cytosolic proteins are N-terminally acetylated, this modification is potentially an untapped target for chemical control of their functions. Structural studies have revealed that, like lysine acetylation, N-terminal acetylation converts a positively charged amine into a hydrophobic handle that mediates protein interactions; hence, this modification may be a ... [more]
Nat. Chem. Biol. Aug. 01, 2017; 13(8);850-857 [Pubmed: 28581483]
Throughput
- High Throughput
Curated By
- BioGRID