An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.

Coleman KE, Bekes M, Chapman JR, Crist SB, Jones MJ, Ueberheide BM, Huang TT

NEDD8 is a ubiquitin-like modifier most well-studied for its role in activating the largest family of ubiquitin E3 ligases, the cullin-RING ligases (CRLs). While many non-cullin neddylation substrates have been proposed over the years, validation of true NEDD8 targets has been challenging, as overexpression of exogenous NEDD8 can trigger NEDD8 conjugation through the ubiquitylation machinery. Here, we developed a deconjugation-resistant ... [more]

Elife Dec. 05, 2016; 6(); [Pubmed: 28475037]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DCUN1D1 UBE2M	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bennett EJ (2010)	High	-	BioGRID	690376
UBE2M DCUN1D1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2219824
UBE2M DCUN1D1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3067965
UBE2M DCUN1D1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	1	BioGRID	3284883
UBE2M DCUN1D1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Scott DC (2017)	High	-	BioGRID	-
DCUN1D1 UBE2M	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Huang G (2014)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Huang G (2014)	Low	-	BioGRID	1051952
DCUN1D1 UBE2M	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Meyer-Schaller N (2009)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Scott DC (2017)	Low	-	BioGRID	-
UBE2M DCUN1D1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhou H (2017)	Low	-	BioGRID	-
UBE2M DCUN1D1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhou W (2019)	Low	-	BioGRID	-
UBE2M DCUN1D1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Kim HS (2021)	Low	-	BioGRID	-
UBE2M DCUN1D1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Scott DC (2011)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Zhou H (2017)	Low	-	BioGRID	2381400
UBE2M DCUN1D1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Kim HS (2021)	Low	-	BioGRID	-
DCUN1D1 UBE2M	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Wang S (2019)	Low	-	BioGRID	2632493
DCUN1D1 UBE2M	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Hammill JT (2018)	Low	-	BioGRID	2600251
DCUN1D1 UBE2M	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Hammill JT (2018)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Zhou H (2017)	Low	-	BioGRID	2381401
UBE2M DCUN1D1	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Zhou W (2019)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Keuss MJ (2016)	Low	-	BioGRID	1521280
DCUN1D1 UBE2M	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kim AY (2008)	Low	-	BioGRID	321859
UBE2M DCUN1D1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kim AY (2008)	Low	-	BioGRID	-
DCUN1D1 UBE2M	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Liu X (2018)	Low	-	BioGRID	2540672
UBE2M DCUN1D1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Monda JK (2013)	Low	-	BioGRID	824229
DCUN1D1 UBE2M	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Scott DC (2017)	Low	-	BioGRID	2484968
DCUN1D1 UBE2M	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhou W (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBE2M

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 transferase activity [IDA]protein binding [IPI]ribosomal S6-glutamic acid ligase activity [IDA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [IMP, TAS]

Gene Ontology Cellular Component

DCUN1D1