SIRT2
Gene Ontology Biological Process
- cellular lipid catabolic process [ISS]
- cellular response to caloric restriction [ISS]
- cellular response to epinephrine stimulus [ISS]
- cellular response to hepatocyte growth factor stimulus [IDA]
- cellular response to hypoxia [IDA]
- cellular response to molecule of bacterial origin [IDA]
- cellular response to oxidative stress [ISS]
- chromatin silencing [NAS]
- chromatin silencing at rDNA [NAS]
- chromatin silencing at telomere [NAS]
- gene silencing [NAS]
- hepatocyte growth factor receptor signaling pathway [IDA]
- histone H3 deacetylation [IMP]
- histone H4 deacetylation [IDA]
- histone deacetylation [IDA, TAS]
- myelination in peripheral nervous system [ISS]
- negative regulation of autophagy [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of defense response to bacterium [IMP]
- negative regulation of fat cell differentiation [ISS]
- negative regulation of oligodendrocyte progenitor proliferation [ISS]
- negative regulation of peptidyl-threonine phosphorylation [ISS]
- negative regulation of protein catabolic process [IMP]
- negative regulation of reactive oxygen species metabolic process [ISS]
- negative regulation of striated muscle tissue development [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- peptidyl-lysine deacetylation [IDA]
- phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of DNA binding [ISS]
- positive regulation of attachment of spindle microtubules to kinetochore [ISS]
- positive regulation of cell division [ISS]
- positive regulation of execution phase of apoptosis [ISS]
- positive regulation of meiosis [ISS]
- positive regulation of oocyte maturation [ISS]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxia [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein ADP-ribosylation [NAS, TAS]
- protein deacetylation [IDA, IMP]
- protein kinase B signaling [IMP]
- regulation of cell cycle [IMP]
- regulation of exit from mitosis [NAS]
- regulation of myelination [ISS]
- regulation of phosphorylation [NAS]
- response to redox state [NAS]
- substantia nigra development [IEP]
- tubulin deacetylation [IDA, IMP, ISS]
Gene Ontology Molecular Function- NAD+ ADP-ribosyltransferase activity [TAS]
- NAD+ binding [IDA]
- NAD-dependent histone deacetylase activity [IDA]
- NAD-dependent histone deacetylase activity (H4-K16 specific) [IDA]
- NAD-dependent protein deacetylase activity [IDA, IMP]
- chromatin binding [IDA]
- histone acetyltransferase binding [IPI]
- histone deacetylase activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein deacetylase activity [IDA, IMP]
- transcription factor binding [IPI]
- tubulin deacetylase activity [IDA]
- ubiquitin binding [IDA]
- zinc ion binding [IDA]
- NAD+ ADP-ribosyltransferase activity [TAS]
- NAD+ binding [IDA]
- NAD-dependent histone deacetylase activity [IDA]
- NAD-dependent histone deacetylase activity (H4-K16 specific) [IDA]
- NAD-dependent protein deacetylase activity [IDA, IMP]
- chromatin binding [IDA]
- histone acetyltransferase binding [IPI]
- histone deacetylase activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein deacetylase activity [IDA, IMP]
- transcription factor binding [IPI]
- tubulin deacetylase activity [IDA]
- ubiquitin binding [IDA]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- Schmidt-Lanterman incisure [ISS]
- centriole [IDA]
- centrosome [IDA]
- chromatin silencing complex [NAS]
- chromosome [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- glial cell projection [ISS]
- juxtaparanode region of axon [ISS]
- lateral loop [ISS]
- meiotic spindle [ISS]
- microtubule [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- myelin sheath [ISS]
- nuclear heterochromatin [ISS]
- nucleus [IDA]
- paranodal junction [ISS]
- paranode region of axon [ISS]
- perikaryon [ISS]
- perinuclear region of cytoplasm [ISS]
- spindle [IDA]
SPOP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SPOP promotes SIRT2 degradation and suppresses non-small cell lung cancer cell growth.
SIRT2 is a NAD-dependent deacetylase and inhibition of SIRT2 has a broad anticancer activity. Here we report that SPOP binds to SIRT2 and mediates its degradation by the 26S proteasome, which can be blocked by MG132 treatment. We also found that the levels of SPOP significantly decreased, while the levels of SIRT2 significantly increased in non-small cell lung cancer (NSCLC) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPOP SIRT2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.448 | BioGRID | 241770 | |
| SPOP SIRT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID